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Image Search Results
Journal: Frontiers in Immunology
Article Title: Metabolic-related gene pairs signature analysis identifies ABCA1 expression levels on tumor-associated macrophages as a prognostic biomarker in primary IDH WT glioblastoma
doi: 10.3389/fimmu.2022.869061
Figure Lengend Snippet: Pharmacological Inhibition of ABCA1 Enhances the Inflammatory Polarization of TAMs In Vivo (A) : Quantification of ABCA1 expression in cholesterol-treated BMDMs ex vivo. BMDMs were treated with the indicated concentrations of cholesterol for 24 h. Cells were harvested and FCM was performed to identify the expression level of ABCA1 (n = 3). (B, C) : Quantification of ABCA1 expression (B) and CD86 and CD206 expression (C) in lovastatin-treated TAMs ex vivo. TAMs were treated with the indicated concentrations of lovastatin for 24 h. FCM was performed to identify these molecular expressions. (D) : Experimental setup of lovastatin-treated murine IDH WT GBM model. Eight mice per group. (E, F) : Representative flow cytometry plots (E) and quantification of ABCA1 expression levels in TAMs (F) . (G–J) : Representative flow cytometry plots (G) and quantification of TAM functional polarization (H–J) . ARG1 (H) were identified as anti-inflammatory macrophage markers; IFN-γ (I) and TNF-α (J) were identified as inflammatory macrophage markers. (K–M) : Experimental setup (K) and tumor growth and survival monitoring (L, M) of lovastatin-treated murine GL261 IDH-WT-Luc model. Eight mice per group. The difference between risk groups was calculated by the Mann–Whitney test and log-rank test (NS, no statistical significance, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).
Article Snippet: Dissociated cells were further incubated with the following antibodies: anti-mouse CD16/CD32 (Multi Sciences, clone 2.4G2, Cat No. AM016-100), IgG2a, κ isotype ctrl (Biolegend, clone MOPC-173, Cat No. 400233), anti-mouse ABCA1 (BIO-RAD, clone 5A1-1422, Cat No. MCA2681), anti-mouse F4/80 (Biolegend, clone BM8, Cat No. 123110), anti-mouse\human CD11b CM1/70, Cat No. 101229), anti-mouse CD86 (Biolegend, clone GL-1, Cat No. 105005), anti-mouse CD206 (Biolegend, clone C068C2, Cat No. 141715), anti-mouse TNF-α (Biolegend, clone MP6-XT22, Cat No. 506303), anti-mouse IFN-γ (Biolegend, clone XMG1.2, Cat No. 506303),
Techniques: Inhibition, In Vivo, Expressing, Ex Vivo, Flow Cytometry, Functional Assay, MANN-WHITNEY